Human/Mouse/Rat p38 gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1347
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human p38 gamma
Accession # P53778
Accession # P53778
Specificity
Detects human, mouse, and rat p38 gamma in Western blots. The antibody is highly specific for p38 gamma and in Western blots, shows extremely weak cross-reactivity with recombinant p38 alpha, p38 beta, or p38 delta after prolonged exposure to film.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat p38 gamma Antibody
Detection of Mouse/Rat p38 gamma by Western Blot.
Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p38 gamma in MCF‑7 Human Cell Line.
p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of human p38 gamma by Western Blot.
Western blot shows recombinant human p38 beta, p38 gamma, p38d and p38a (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approx-imately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human/Mouse/Rat p38 gamma Antibody
Application
Recommended Usage
Immunocytochemistry
3-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast, human skin cancer tissue, and mouse skeletal muscle
Sample: Immersion fixed paraffin-embedded sections of human breast, human skin cancer tissue, and mouse skeletal muscle
Simple Western
2 µg/mL
Sample: C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line
Sample: C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line
Western Blot
0.2 µg/mL
Sample: PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line
Sample: PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 gamma
down-regulation of cyclin D1 in response to hypoxia in adrenal cells.
Additional p38 gamma Products
Product Documents for Human/Mouse/Rat p38 gamma Antibody
Product Specific Notices for Human/Mouse/Rat p38 gamma Antibody
For research use only
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