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Human/Mouse/Rat PAK4 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4178

R&D Systems, part of Bio-Techne
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AF4178
AF4178-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human PAK4
Met1-Glu120
Accession # O96013

Specificity

Detects endogenous human, mouse and rat PAK4 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PAK4 Antibody

Detection of Human/Mouse/Rat PAK4 antibody by Western Blot.

Detection of Human/Mouse/Rat PAK4 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, PT18 mouse mast/basophil cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat PAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4178) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PAK4 at approximately 72 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human PAK4 by Simple WesternTM.

Simple Western shows lysates of MCF-7 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for PAK4 at approximately 66 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat PAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4178). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human/Mouse/Rat PAK4 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: MCF-7 human breast cancer cell line

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, PT18 mouse mast/basophil cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAK4

As downstream targets of the Rho GTPases, the p21-activated kinase (PAK) family of serine/threonine kinases regulates the organization of the actin cytoskeleton in mammalian cells. The PAK family is structurally categorized in two groups, each with three members: group I PAK1-3; and group II PAK4-6. Targeted disruption of PAK4 in mice leads to severe defects in the development and migration of neurons, suggesting a key role for this kinase in neuronal development.

Long Name

p21/Cdc42/Rac1-activated Kinase 4

Alternate Names

EC 2.7.11, EC 2.7.11.1, KIAA1142, p21 protein (Cdc42/Rac)-activated kinase 4, p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, PAK-4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, serine/threonine-protein kinase PAK 4

Entrez Gene IDs

10298 (Human); 70584 (Mouse); 292756 (Rat)

Gene Symbol

PAK4

UniProt

Additional PAK4 Products

Product Documents for Human/Mouse/Rat PAK4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat PAK4 Antibody

For research use only

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