Human/Mouse/Rat Pannexin-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7097

R&D Systems, part of Bio-Techne
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MAB7097
MAB7097-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 720505

View all Pannexin-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pannexin-1
Pro298-Asp376
Accession # Q96RD7

Specificity

Detects human Pannexin-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Pannexin-2 or -3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Pannexin-1 Antibody

Detection of Human, Mouse, and Rat Pannexin-1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Pannexin‑1 by Western Blot.

Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Pannexin-1 Monoclonal Antibody (Catalog # MAB7097) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pannexin-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Pannexin-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain tissue, mouse brain tissue, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pannexin-1

Pannexin-1 (PANX1) is an approximately 45 kDa member of the Pannexin family of four-transmembrane channel proteins with a conserved pattern of cysteines. It is expressed on epithelial cells, neuronal and glial cells, cardiac myocytes, T cells, and erythrocytes. Pannexin-1 forms large pore hexameric channels or heteromeric channels with Pannexin-2 and enables the efflux of ATP and UTP. Its conductance is increased in response to hypoxia, isotonic stress, NMDA R activation, and Caspase-3 and -7 activation during apopotosis. Extracellular release of ATP through Pannexin-1 activates P2X7 receptors which in turn induces the closure of Pannexin-1 channels. Pannexin-1 cooperates with P2X7 for inflammasome activation, Caspase-1 activation, and the release of mature IL-1 beta and also regulates T cell activation at the immunological synapse. Within aa 298-376 (in the C-terminal cytoplasmic domain), human Pannexin-1 shares 96% and 98% aa sequence identity with mouse and rat Pannexin-1, respectively. Alternate splicing generates an additional isoform that lacks the C-terminal four amino acids in the cytoplasmic tail.

Alternate Names

Innexin, MRS1, Pannexin1, PANX1, PX1

Entrez Gene IDs

24145 (Human); 55991 (Mouse); 315435 (Rat)

Gene Symbol

PANX1

UniProt

Q96RD7

Additional Pannexin-1 Products

Product Documents for Human/Mouse/Rat Pannexin-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Pannexin-1 Antibody

For research use only

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