Human/Mouse/Rat PDCD6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6015

R&D Systems, part of Bio-Techne
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MAB6015
MAB6015-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 722232

View all PDCD6 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PDCD6
Met1-Val189
Accession # O75340

Specificity

Detects human PDCD6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) PDCD4 or rhPDCD5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat PDCD6 Antibody

Detection of Human, Mouse, and Rat PDCD6 antibody by Western Blot.

Detection of Human, Mouse, and Rat PDCD6 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat PDCD6 Monoclonal Antibody (Catalog # MAB6015) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PDCD6 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and NRK rat normal kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDCD6

PDCD6 (Programmed cell death protein 6; also ALG-2 and apoptosis-linked gene 2 protein) is a 22 kDa member of the penta-EF hand protein family of molecules. It is ubiquitously expressed, and performs multiple functions. PDCD6 is contextually linked to apoptosis, proliferation, membrane fusion and vesicle trafficking. Human PDCD6 is 191 amino acids (aa) in length. Although it contains five EF-hand domains, only domains #1 and #3 are considered functional (aa 23-58 and 90‑125). There is one splice form (ALG-2.1) that shows a deletion of Gly121Phe122. This form typically represents about 1/3 of total cellular PDCD6. PDCD6/ALG-2 will homodimerize, and heterodimerize with ALG-2.1. Notably, there are classes of cytosolic proteins that bind PDCD6, but not ALG-2.1, accounting for differences in function. Both human PDCD6 isoforms share 99% aa identity with their mouse counterparts.

Long Name

Programmed Cell Death 6

Alternate Names

ALG2, PEF1B

Entrez Gene IDs

10016 (Human)

Gene Symbol

PDCD6

UniProt

O75340 (Human)

Additional PDCD6 Products

Product Documents for Human/Mouse/Rat PDCD6 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PDCD6 Antibody

For research use only

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