Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8407
Key Product Details
Validated by
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody
Detection of Human Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) Monoclonal Antibody (Catalog # MAB8407) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody
Western Blot
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line treated with PMA or PMA and Ionomycin
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEK1/MEK2
MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks, including the Raf kinases. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.
Long Name
Alternate Names
Additional MEK1/MEK2 Products
Product Documents for Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody
Product Specific Notices for Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only