Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8965
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1143C
Product Specifications
Immunogen
Phosphopeptide containing the human p70 S6 Kinase T421/S424 site
Specificity
Detects human p70 S6 Kinase when phosphorylated at T421/S424.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antibody
Detection of Human Phospho-p70 S6 Kinase by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 1 hour. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Monoclonal Antibody (Catalog # MAB8965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phopsho-p70 S6 Kinase (T421/S424) at approximately 70 kDa and Phospho-p85 S6 Kinase (T444/S447) at 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1)
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p70 S6 Kinase
References
- Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
- Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
Alternate Names
p70-alpha, RPS6KB1, S6K1, STK14A
Gene Symbol
RPS6KB1
Additional p70 S6 Kinase Products
Product Specific Notices for Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antibody
For research use only
Loading...
Loading...
Loading...
Loading...