Human/Mouse/Rat Phospho-PLC-gamma 1 (Y783) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB74541
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1016A
Product Specifications
Immunogen
Phosphopeptide containing the human/mouse/rat PLC-gamma 1 Y783 site
Accession # P19174
Accession # P19174
Specificity
Detects human/mouse/rat PLC-gamma 1 when phosphorylated at Y783 in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Phospho-PLC-gamma 1 (Y783) Antibody
Detection of Human Phospho-PLC‑ gamma1 (Y783) by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PLC- gamma1 (Y783) Monoclonal Antibody (Catalog # MAB74541) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PLC- gamma1 (Y783) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Phospho-PLC-gamma 1 (Y783) Antibody
Application
Recommended Usage
Western Blot
0.05 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate (PV)
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate (PV)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 1
Long Name
Phospholipase C gamma 1
Alternate Names
PLCG1, PLCgamma 1, PPLCA
Gene Symbol
PLCG1
UniProt
Additional PLC-gamma 1 Products
Product Documents for Human/Mouse/Rat Phospho-PLC-gamma 1 (Y783) Antibody
Product Specific Notices for Human/Mouse/Rat Phospho-PLC-gamma 1 (Y783) Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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