Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF892
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Canine
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human RSK1 S221 site
Specificity
Detects human, mouse, and rat RSK1 when phosphorylated at S221, and human and mouse RSK2 when phosphorylated at S227.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody
RSK1/RSK2 in Mouse Brain.
RSK1/RSK2 was detected in perfusion fixed frozen sections of mouse brain (Purkinje cells in cerebellum) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human Phospho-RSK1 (S221) and RSK2 (S227) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S221) and RSK2 (S227) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (Purkinje cells in cerebellum)
Sample: Perfusion fixed frozen sections of mouse brain (Purkinje cells in cerebellum)
Western Blot
0.5 µg/mL
Sample: PMA-treated HeLa human cervical epithelial carcinoma cell line
Sample: PMA-treated HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: RSK1/RSK2
Long Name
Ribosomal Protein S6 Kinase II
Additional RSK1/RSK2 Products
Product Documents for Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody
Product Specific Notices for Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody
For research use only
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