Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2998
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 572106
Product Specifications
Immunogen
E. coli-derived recombinant human PI 3-Kinase p85 alpha
aa Lys519-Gly644
Accession # P27986
aa Lys519-Gly644
Accession # P27986
Specificity
Detects human, mouse and rat PI 3-Kinase p85 alpha in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line, K562 human chronic myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PI 3-Kinase p85a Monoclonal Antibody (Catalog # MAB2998) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p85a at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PI 3-Kinase p85 alpha by Simple WesternTM.
Simple Western lane view shows lysates of A172 human glioblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PI 3‑Kinase p85 alpha at approximately 89 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat PI 3‑Kinase p85 alpha Monoclonal Antibody (Catalog # MAB2998). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: A172 human glioblastoma cell line
Sample: A172 human glioblastoma cell line
Western Blot
1 µg/mL
Sample: A172 human glioblastoma cell line, K562 human chronic myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: A172 human glioblastoma cell line, K562 human chronic myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p85 alpha
Long Name
PI 3-Kinase p85 alpha
Alternate Names
GRB1, PI 3Kinase p85 alpha, PIK3R1
Entrez Gene IDs
5295 (Human)
Gene Symbol
PIK3R1
UniProt
Additional PI 3-Kinase p85 alpha Products
Product Documents for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
Product Specific Notices for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
For research use only
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