Human/Mouse/Rat PON3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4345
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 454929
Product Specifications
Immunogen
E. coli-derived recombinant human PON3
Ala30-Leu354
Accession # AAH70374
Ala30-Leu354
Accession # AAH70374
Specificity
Detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON1 or rhPON2.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat PON3 Antibody
Detection of Human/Mouse/Rat PON3 by Western Blot.
Western blot shows lysates of human liver tissue, an enriched HDL /LDL sample obtained from ultracentrifugation of human plasma, mouse liver, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat PON3 Monoclonal Antibody (Catalog # MAB4345) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat PON3 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Human liver tissue, an enriched HDL /LDL sample obtained from ultracentrifugation of human plasma, mouse liver, and rat liver tissue
Sample: Human liver tissue, an enriched HDL /LDL sample obtained from ultracentrifugation of human plasma, mouse liver, and rat liver tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PON3
Long Name
Paraoxonase 3
Alternate Names
EC 3.1.1.2, EC 3.1.1.81, EC 3.1.8.1, paraoxanase-3, paraoxonase 3, serum paraoxonase/lactonase 3
Gene Symbol
PON3
UniProt
Additional PON3 Products
Product Documents for Human/Mouse/Rat PON3 Antibody
Product Specific Notices for Human/Mouse/Rat PON3 Antibody
For research use only
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