Human/Mouse/Rat PP1 Catalytic Subunit Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3000
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Bovine
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 319319
Product Specifications
Immunogen
E. coli-derived recombinant human PP1 alpha catalytic subunit
Lys41-Pro298
Accession # P62136
Lys41-Pro298
Accession # P62136
Specificity
Detects endogenous human, mouse and rat PP1 alpha. Based on the amino acid homologies, the antibody is expected to cross‑react with PP1 beta and PP1 gamma.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat PP1 Catalytic Subunit Antibody
Detection of Human/Mouse/Rat PP1ca by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Nb2-11 rat lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP1 Catalytic Subunit Monoclonal Antibody (Catalog # MAB3000) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PP1 catalytic subunits at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PP1 Catalytic Subunit Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Nb2-11 rat lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Nb2-11 rat lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP1
Protein Phosphatase 1, also known as PP1 and PPP1, is an enzyme that removes phosphate groups attached to serine or threonine residues in proteins. The holoenzyme is composed of two subunits, a catalytic subunit (PP1ca) that is highly conserved throughout evolution, and a wide variety of regulatory subunits that target the enzyme to specific subcellular compartments and proteins. The catalytic subunit is ubiquitously expressed in tissues and cell lines.
Long Name
Protein Phosphatase 1
Alternate Names
PP1A;PP-1A;PP1alpha;PPP1A;PPP1CA;Serine/threonine-protein phosphatase;Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Gene Symbol
PPP1CA
UniProt
Additional PP1 Products
Product Documents for Human/Mouse/Rat PP1 Catalytic Subunit Antibody
Product Specific Notices for Human/Mouse/Rat PP1 Catalytic Subunit Antibody
For research use only
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