Human/Mouse/Rat Rap2A/B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4698
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 455410
Product Specifications
Immunogen
E. coli-derived recombinant human Rap2A/B
Met1-Gln183
Accession # P10114
Met1-Gln183
Accession # P10114
Specificity
Detects human, mouse, and rat Rap2A/B in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat Rap2A/B Antibody
Detection of Human, Mouse, and Rat Rap2A/B by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat Rap2A/B Monoclonal Antibody (Catalog # MAB4698) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Rap2A and Rap2B (5 ng/lane) were included. A specific band for Rap2A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat Rap2A/B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line
Sample: MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rap2A/B
Rap2A and Rap2B are members of the small GTPase superfamily and ras family of proteins. Both proteins are 183 amino acids (aa) in length (SwissProt P10114 and P61225), and they are 90% identical at the amino acid level, with the most variability at the carboxyl terminus of the protein. Unlike normal Ras proteins that have a nontransforming glutamine at aa 61, these two oncogenic proteins have a threonine in that position. This change makes the intrinsic GTPase activity for these proteins lower, thereby allowing them to exist in the activated state for a longer period of time than normal Ras proteins.
Long Name
Ras-related Protein 2A/B
Alternate Names
KREV;K-REV;RAP2;Ras-related protein Rap-2a;RbBP-30
UniProt
Additional Rap2A/B Products
Product Documents for Human/Mouse/Rat Rap2A/B Antibody
Product Specific Notices for Human/Mouse/Rat Rap2A/B Antibody
For research use only
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