Human/Mouse/Rat RSK Pan Specific Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2056
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Human RSK2 synthetic peptide
Specificity
Detects endogenous human, mouse and rat RSK family isoforms in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat RSK Pan Specific Antibody
Detection of Human and Mouse RSK by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat RSK Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2056) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RSK Pan Specific, at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat RSK Pan Specific Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RSK
Long Name
Ribosomal Protein S6 Kinase II
Alternate Names
HU-1;MAPKAPK1;MAPKAPK1A;p90Rsk;Ribosomal protein S6 kinase alpha-1;RPS6KA1;RSK;RSK1
Additional RSK Products
Product Documents for Human/Mouse/Rat RSK Pan Specific Antibody
Product Specific Notices for Human/Mouse/Rat RSK Pan Specific Antibody
For research use only
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