Human/Mouse/Rat SIRP delta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB101381

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB101381-100
MAB101381-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2588B

View all SIRP delta Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human SIRP delta
Phe30-Arg197
Accession # Q9H106

Specificity

Detects human SIRP delta in direct ELISAs. Detects human, mouse, and rat SIRP delta in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat SIRP delta Antibody

Detection of Human, Mouse, and Rat SIRP delta antibody by Western Blot.

Detection of Human, Mouse, and Rat SIRP delta by Western Blot.

Western blot shows lysates of human testis tissue, mouse testis tissue, and rat testis tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat SIRP delta Monoclonal Antibody (Catalog # MAB101381) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody HAF008. A specific band was detected for SIRP delta at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using our Western Blot Buffer Group 1.

SIRP delta in THP-1 Human Cell Line.

SIRP delta was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel; positive staining) and MCF-7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human/Mouse/Rat SIRP delta Monoclonal Antibody (Catalog # MAB101381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

SIRP delta in U937 Human Cell Line.

SIRP delta was detected in immersion fixed U937 human histiocytic lymphoma cell line (left panel; positive staining) and MCF-7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human/Mouse/Rat SIRP delta Monoclonal Antibody (Catalog # MAB101381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol forFluorescent ICC Staining of Non-adherent Cells.

Applications for Human/Mouse/Rat SIRP delta Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line and immersion fixed U937 human histiocytic lymphoma cell line

Western Blot

2 µg/mL
Sample: Human testis tissue, Mouse testis tissue, and Rat testis tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP delta

SIRP delta (SIRPD; Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

References

  1. Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
  2. Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
  3. Huttlin, E.L. et al. (2017) Nature 545:505.

Long Name

Signal-regulatory Protein delta

Alternate Names

dJ576H24.4, PTPNS1L2, SIRP-Delta, SIRPD

Entrez Gene IDs

128646 (Human)

Gene Symbol

SIRPD

UniProt

Q9H106

Additional SIRP delta Products

Product Documents for Human/Mouse/Rat SIRP delta Antibody

Product Datasheets

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Product Specific Notices for Human/Mouse/Rat SIRP delta Antibody

For research use only

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