Human/Mouse/Rat Src Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3389
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 327537
Product Specifications
Immunogen
E. coli-derived recombinant human Src
Met1-Ala79
Accession # P12931
Met1-Ala79
Accession # P12931
Specificity
Detects endogenous human, mouse and rat Src in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat Src Antibody
Detection of Human and Rat Src by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, Y3-Ag rat myeloid cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Src Monoclonal Antibody (Catalog # MAB3389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Src at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human Src by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HL-60 human acute promyelocytic leukemia cell line (negative control). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Src Monoclonal Antibody (Catalog # MAB3389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Src at approximately 60 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human/Mouse/Rat Src Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line, Y3-Ag rat myeloid cell line, Rat-2 rat embryonic fibroblast cell line, A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Sample: MCF-7 human breast cancer cell line, Y3-Ag rat myeloid cell line, Rat-2 rat embryonic fibroblast cell line, A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Src
The Src family of proteins are intracellular tyrosine kinases involved in cell proliferation, differentiation, motility, and survival. Src family activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Autophosphorylation in the activation loop of the kinase domain (Y419 of human Src) upregulates the enzyme, while phosphorylation in the C-terminal tail (Y530 of human Src) downregulates activity.
Long Name
v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog
Alternate Names
ASV, c-Src, RSVgp4
Gene Symbol
SRC
UniProt
Additional Src Products
Product Documents for Human/Mouse/Rat Src Antibody
Product Specific Notices for Human/Mouse/Rat Src Antibody
For research use only
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