Human/Mouse/Rat Syntaxin-BP1 Antibody

Detection of Syntaxin-BP1 by Immunoprecipitation.

U-87 MG human glioblastoma/astrocytoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Goat Anti-Human/Mouse/Rat Syntaxin-BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) pre-coupled to Dynabeads Protein G. Immunoprecipitated Syntaxin-BP1 was detected in Western Blot with a Rabbit Syntaxin-BP1 antibody. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Syntaxin-BP1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

U-87 MG human glioblastoma/astrocytoma parental cell line WT and Syntaxin-BP1 U-87 MG KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Goat Anti-Human/Mouse/Rat Syntaxin-BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 1 ug/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).