Human/Mouse/Rat TPT1/TCTP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4648
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # 488411
Product Specifications
Immunogen
E. coli-derived recombinant human TPT1/TCTP
Met1-Cys172
Accession # P13693
Met1-Cys172
Accession # P13693
Specificity
Detects human, mouse, and rat TPT1/TCTP in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat TPT1/TCTP Antibody
Detection of Human/Mouse/Rat TPT1/TCTP by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, Y3-Ag rat myeloid cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat TPT1/TCTP Monoclonal Antibody (Catalog # MAB4648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human TPT1 (5 ng) was included. A specific band for TPT1/TCTP was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
TPT1/TCTP in HeLa Human Cell Line.
TPT1/TCTP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line cell line using Mouse Anti-Human/Mouse/Rat TPT1/TCTP Monoclonal Antibody (Catalog # MAB4648) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse/Rat TPT1/TCTP Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, Y3-Ag rat myeloid cell line, and C2C12 mouse myoblast cell line
Sample: Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, Y3-Ag rat myeloid cell line, and C2C12 mouse myoblast cell line
Reviewed Applications
Read 2 reviews rated 4.5 using MAB4648 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TPT1/TCTP
Long Name
Translationally-controlled Tumor Protein 1
Alternate Names
Fortilin, HRF, p02, TCTP
Gene Symbol
TPT1
UniProt
Additional TPT1/TCTP Products
Product Documents for Human/Mouse/Rat TPT1/TCTP Antibody
Product Specific Notices for Human/Mouse/Rat TPT1/TCTP Antibody
For research use only
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