Human/Mouse SALL4 Antibody

Name: Human/Mouse SALL4 Antibody
Brand: R&D Systems
SKU: MAB6374
Rating: 5 (2 reviews)

R&D Systems, part of Bio-Techne | Catalog # MAB6374

(2)

Citations (1)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 651839

View all SALL4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SALL4
Lys96-Gly359
Accession # Q9UJQ4

Specificity

Detects endogenous human and mouse SALL4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Mouse SALL4 Antibody

Detection of Human SALL4 by Western Blot.

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.025 µg/mL of Mouse Anti-Human/Mouse SALL4 Monoclonal Antibody (Catalog # MAB6374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SALL4 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse SALL4 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis

Western Blot

0.025 µg/mL
Sample: BJAB human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB6374 in the following applications:

Immunohistochemistry (1 Review)
Simple Western (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALL4

SALL4 (SAL-like protein 4) is a 1324 amino acid (aa) member of the SAL protein family that possess multiple C₂H₂-type zinc-fingers. It is a transcriptional repressor when associated with histone deacetylase and a transcriptional activator of the Wnt pathway in its native form. It contributes to generation of induced pluripotent stem cells and to axis formation during development. It is frequently overexpressed in germ cell tumors and acute myeloid leukemias. Mutations of human SALL4 can result in conditions such as Okihiro, Duane Radial Ray, or Holt-Oram syndromes. The region used as an immunogen is common to both SALL4 and the ubiquitous 617 aa isoform, SALL4B; it shares 76% aa identity between human and mouse.

Long Name

Sal-like 4

Alternate Names

DRRS, HSAL4, ZNF797

Entrez Gene IDs

57167 (Human); 99377 (Mouse)

Gene Symbol

SALL4

UniProt

Q9UJQ4 (Human)

Additional SALL4 Products

Product Documents for Human/Mouse SALL4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse SALL4 Antibody

For research use only

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