Human/Mouse SOCS-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5628

R&D Systems, part of Bio-Techne
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MAB5628
MAB5628-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 517331

View all SOCS-4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOCS-4
Met1-Cys440
Accession # Q8WXH5

Specificity

Detects endogenous human and mouse SOCS-4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse SOCS-4 Antibody

Detection of Human/Mouse SOCS-4 antibody by Western Blot.

Detection of Human/Mouse SOCS-4 by Western Blot.

Western blot shows lysates of NC-37 human Burkitt's lymphoma B lymphoblast cell line, HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse SOCS-4 Monoclonal Antibody (Catalog # MAB5628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-4 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse SOCS-4 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: NC-37 human Burkitt's lymphoma B lymphoblast cell line, HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-4

Suppressor of cytokine signaling 4 (SOCS-4) is a 51 kDa (predicted) member of the SOCS family of proteins, which are Src homology-2 (SH2)-containing proteins originally identified as negative regulators of cytokine signaling. Human SOCS-4 is 440 amino acids (aa) in length. It contains an SH2 domain (aa 289‑381) and a SOCS box domain (aa 376‑425). Human SOCS-4 shares 93% and 86% aa sequence identity with bovine and mouse SOCS-4, respectively. The biological function of SOCS-4 remains poorly understood, but it has been found to be elevated in cells following treatment with EGF, acting perhaps in a feedback loop inhibiting STAT3.

Long Name

Suppressor Of Cytokine Signaling 4

Alternate Names

CIS4, SOCS-7, SOCS4, SSI4

Entrez Gene IDs

122809 (Human); 67296 (Mouse); 305828 (Rat)

Gene Symbol

SOCS4

UniProt

Q8WXH5

Additional SOCS-4 Products

Product Documents for Human/Mouse SOCS-4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse SOCS-4 Antibody

For research use only

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