Human/Mouse Tec Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6519
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 663921
Product Summary for Human/Mouse Tec Antibody
Immunogen
E. coli-derived recombinant human Tec
Met1-Met187
Accession # P42680
Met1-Met187
Accession # P42680
Specificity
Detects human Tec in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse Tec Antibody
Detection of Human and Mouse Tec by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and BaF3 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Human/Mouse Tec Monoclonal Antibody (Catalog # MAB6519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tec at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Tec in Human Liver.
Tec was detected in immersion fixed paraffin-embedded sections of human liver using Human/Mouse Tec Monoclonal Antibody (Catalog # MAB6519) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse Tec Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Western Blot
1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and BaF3 mouse pro-B cell line
Sample: Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and BaF3 mouse pro-B cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tec
The tyrosine protein kinase Tec is an approximately 70 kDa member of the protein kinase superfamily, tyrosine protein family, and Tec subfamily. Human Tec is 631 amino acids (aa) in length. Amino acid residues 4-111 constitute a Tec pleckstrin homology (PH) domain, and aa 113-149 make up a Btk-type zinc finger. Residues 179-239 form a Src homology 3 (SH3) domain, and aa 247-345 make up a Src homology 2 (SH2) domain. The region involving positions 365-620 constitutes the catalytic domain of the protein tyrosine kinases. Human Tec shares 94% aa sequence identity with mouse Tec. Human Tec is expressed in hematopoietic cell lines including B-cell, T-cell, and myeloid lineages.
Long Name
Translocated in Extraskeletal Chondrosarcoma Protein Tyrosine Kinase
Alternate Names
PSCTK4
Entrez Gene IDs
7006 (Human)
Gene Symbol
TEC
UniProt
Additional Tec Products
Product Documents for Human/Mouse Tec Antibody
Product Specific Notices for Human/Mouse Tec Antibody
For research use only
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