Human/Mouse TRANCE/TNFSF11/RANK L Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6263
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 685809
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
Gly64-Asp245
Accession # AAC51762
Gly64-Asp245
Accession # AAC51762
Specificity
Detects human TRANCE/TNFSF11/RANK L in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) TRANCE and approximately 25% cross-reactivity with recombinant human (rh) LIGHT is observed. No cross-reactivity with rh4-1BB Ligand, rhAPRIL, rhBAFF, rhEDA, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhOX40 LIgand, rhTNF-alpha, rhTRAIL, rhTWEAK, or rhVEGI is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human/Mouse TRANCE/TNFSF11/RANK L Antibody
Detection of Human TRANCE/TNFSF11/RANK L by Western Blot.
Western blot shows lysates of human primary differentiated CD4+Th2 cells. PVDF Membrane was probed with 2 µg/mL of Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB6263) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRANCE/TNFSF11/RANK L at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse TRANCE/TNFSF11/RANK L Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human primary differentiated CD4+ Th2 cells
Sample: Human primary differentiated CD4+ Th2 cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRANCE/TNFSF11/RANK L
Long Name
TNF-related Activation-induced Cytokine
Alternate Names
CD254, ODF, OPGL, RANK L, RANKL, TNFSF11
Gene Symbol
TNFSF11
UniProt
Additional TRANCE/TNFSF11/RANK L Products
Product Documents for Human/Mouse TRANCE/TNFSF11/RANK L Antibody
Product Specific Notices for Human/Mouse TRANCE/TNFSF11/RANK L Antibody
For research use only
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