Human/Mouse UBE2K/E2-25K Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6609
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 701316
Product Specifications
Immunogen
E. coli-derived recombinant human UBE2K/E2‑25K
Ala2-Asn200
Accession # P61086
Ala2-Asn200
Accession # P61086
Specificity
Detects human UBE2K/E2‑25K in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse UBE2K/E2-25K Antibody
Detection of Human UBE2K/E2‑25K by Western Blot.
Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human UBE2K/E2-25K Monoclonal Antibody (Catalog # MAB6609) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UBE2K/E2-25K at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
UBE2K/E2‑25K in Human Brain.
UBE2K/ E2-25K was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human UBE2K/E2-25K Monoclonal Antibody (Catalog # MAB6609) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse UBE2K/E2-25K Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Western Blot
2 µg/mL
Sample: Human brain (cortex) tissue
Sample: Human brain (cortex) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UBE2K/E2-25K
Long Name
Ubiquitin-conjugating Enzyme E2
Alternate Names
E2-25K, E225K, Hip2, HYPG, LIG, UbcH1, UBE2K, UBE2K-25K
Gene Symbol
UBE2K
UniProt
Additional UBE2K/E2-25K Products
Product Documents for Human/Mouse UBE2K/E2-25K Antibody
Product Specific Notices for Human/Mouse UBE2K/E2-25K Antibody
For research use only
Loading...
Loading...
Loading...
Loading...