Human/Mouse ZIC1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4978
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ZIC1
Met1-Gly198
Accession # Q15915
Met1-Gly198
Accession # Q15915
Specificity
Detects human and mouse ZIC1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse ZIC1 Antibody
Detection of Human/Mouse ZIC1 by Western Blot.
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ZIC1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
ZIC1 in Mouse Brain.
ZIC1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
ZIC1 in Human iPSK3 cells.
ZIC1 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human/Mouse ZIC1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3)
Sample: Immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3)
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse brain (cerebellum)
Sample: Immersion fixed frozen sections of mouse brain (cerebellum)
Western Blot
1 µg/mL
Sample: Human brain tissue
Sample: Human brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZIC1
Long Name
Zic Family Member 1
Alternate Names
ZNF201
Gene Symbol
ZIC1
UniProt
Additional ZIC1 Products
Product Documents for Human/Mouse ZIC1 Antibody
Product Specific Notices for Human/Mouse ZIC1 Antibody
For research use only
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