Human MRGPRF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8396
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 916219
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human MRGPRF
Met1-Ser343
Accession # NP_659452
Met1-Ser343
Accession # NP_659452
Specificity
Detects HEK293 human embryonic kidney cell line transfected with human MRGPRF by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MRGPRF Antibody
Detection of Human MRGPRF by Western Blot.
Western blot shows lysates of human uterus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MRGPRF at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of MRGPRF in HEK293 Human Cell Line Transfected with Human MRGPRF and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human MRGPRF or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002).Applications for Human MRGPRF Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human MRGPRF and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human MRGPRF and eGFP
Western Blot
2 µg/mL
Sample: Human uterus tissue
Sample: Human uterus tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MRGPRF
References
- Hohenhaus D.M., et al. (2013) Immunobiology 218:1345.
Long Name
Mas-related G Protein-coupled Receptor Member F
Alternate Names
GPR140, GPR168, RTA
Gene Symbol
MRGPRF
UniProt
Additional MRGPRF Products
Product Documents for Human MRGPRF Antibody
Product Specific Notices for Human MRGPRF Antibody
For research use only
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