Human Myeloperoxidase/MPO Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3174
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Gerbil
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
ELISA Capture, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 392105
Product Specifications
Immunogen
E. coli-derived recombinant human Myeloperoxidase/MPO
Val279-Ser745
Accession # P05164
Val279-Ser745
Accession # P05164
Specificity
Detects human Myeloperoxidase/MPO in Western blots. No cross-reactivity with recombinant human Eosinophil Peroxidase is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Myeloperoxidase/MPO Antibody
Detection of Human Myeloperoxidase/MPO by Western Blot.
Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and human neutrophil. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myeloperoxidase/MPO at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Myeloperoxidase/MPO in MOLT-4 Human Cell Line.
Myeloperoxidase/ MPO was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using 8 µg/mL Mouse Anti-Human Myeloperoxidase/ MPO Monoclonal Antibody (Catalog # MAB3174) for 3 hours at room temperature. Cells were stained (red) and counter-stained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Myeloperoxidase/MPO in HL‑60 Human Cell Line.
Myeloperoxidase/MPO was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Myeloperoxidase/MPO Mono-clonal Antibody (Catalog # MAB3174) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Myeloperoxidase/MPO Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line and HL-60 human acute promyelocytic leukemia cell line
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line and HL-60 human acute promyelocytic leukemia cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen and mouse spleen
Sample: Immersion fixed paraffin-embedded sections of human spleen and mouse spleen
Western Blot
1 µg/mL
Sample: HL-60 human acute promyelocytic leukemia cell line and human neutrophil
Sample: HL-60 human acute promyelocytic leukemia cell line and human neutrophil
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 3.4 using MAB3174 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myeloperoxidase/MPO
Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta-subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta-subunits and two alpha-subunits that are also disulfide-linked through the two alpha-subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.
Alternate Names
MPO
Gene Symbol
MPO
UniProt
Additional Myeloperoxidase/MPO Products
Product Documents for Human Myeloperoxidase/MPO Antibody
Product Specific Notices for Human Myeloperoxidase/MPO Antibody
For research use only
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