Human Myocilin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2537
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Myocilin
Arg33-Met504
Accession # Q99972
Arg33-Met504
Accession # Q99972
Specificity
Detects human Myocilin in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Myocilin Antibody
Detection of Human Myocilin by Western Blot.
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Myocilin at approximately 55 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Myocilin by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Myocilin at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Myocilin Antibody
Application
Recommended Usage
Simple Western
50 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Western Blot
1 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myocilin
Additional Myocilin Products
Product Documents for Human Myocilin Antibody
Product Specific Notices for Human Myocilin Antibody
For research use only
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