Human Myoglobin Antibody

Name: Human Myoglobin Antibody
Brand: R&D Systems
SKU: MAB97204
Rating: 5 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # MAB97204

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2269D

View all Myoglobin Antibodies »

Product Specifications

Immunogen

Purified human Myoglobin antigen from human heart
Accession # P02144

Specificity

Detects human Myoglobin in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Myoglobin Antibody

Myoglobin in Human Heart.

Myoglobin was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97204) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Myoglobin by Western Blot.

Western blot shows lysates of human heart tissue and human liver tissue (negative control). PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97204) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Myoglobin at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human Myoglobin ELISA Standard Curve.

Recombinant Human Myoglobin protein was serially diluted 2-fold and captured by Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97204) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human/Mouse/Rat Myoglobin Monoclonal Antibody (Catalog # MAB97203) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Myoglobin Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human/Mouse/Rat Myoglobin Monoclonal Antibody (Catalog # MAB97203).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Western Blot

0.05 µg/mL
Sample: Human heart tissue

Reviewed Applications

Read 1 review rated 5 using MAB97204 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myoglobin

Myoglobin is a 17KDa cytoplasmic oxygen-binding protein encoded by the MB gene and expressed in myocytes of the heart and skeletal muscle. Its name derives from its structural and functional similarity to hemoglobin, the oxygen binding protein found in red blood cells. Functions of myoglobin include oxygen storage and transport, as well as scavenging of NO and reactive oxygen species. Myoglobin also serves as a sensitive marker for muscle injury resulting from cardiac infarction. Myoglobin was the first protein to have its three-dimensional structure determined by X-ray crystallography.