Human NAIP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB829
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 541609
Product Specifications
Immunogen
E.coli-derived recombinant human NAIP
Asn923-Val1148
Accession # Q13075
Asn923-Val1148
Accession # Q13075
Specificity
Detects human NAIP in Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) NAIP or rmNAIP2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human NAIP Antibody
Detection of Human NAIP by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NAIP and human brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Human NAIP Monoclonal Antibody (Catalog # MAB829) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NAIP at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
NAIP in Human Brain.
NAIP was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Human NAIP Monoclonal Antibody (Catalog # MAB829) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human NAIP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human NAIP and human brain (cerebellum) tissue
Sample: HEK293 human embryonic kidney cell line transfected with human NAIP and human brain (cerebellum) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NAIP
Long Name
Neuronal Apoptosis Inhibitor Protein
Alternate Names
BIRC1, NLRB1
Gene Symbol
NAIP
UniProt
Additional NAIP Products
Product Documents for Human NAIP Antibody
Product Specific Notices for Human NAIP Antibody
For research use only
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