Human NAIP Antibody

R&D Systems, part of Bio-Techne | Catalog # AF829

R&D Systems, part of Bio-Techne
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AF829
AF829-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all NAIP Antibodies »

Product Specifications

Immunogen

KLH-coupled human NAIP synthetic peptide
YLSLSSTRPDEGLASIIC
Accession # NP_004527

Specificity

Detects human NAIP expressed in Sf 21 insect cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human NAIP Antibody

Detection of Human NAIP antibody by Western Blot.

Detection of Human NAIP by Western Blot.

Western blot shows lysates ofSf21 insect cells before (lane 1) and 48 hours post infection with human NAIP (lane 2). PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human NAIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF829) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for truncated NAIP was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human NAIP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Sf 21 insect cells infected with human NAIP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAIP

Neuronal apoptosis inhibitory protein (NAIP; also Baculoviral IAP repeat-containing protein 1) is a 160 kDa member of the inhibitor of apoptosis family of proteins (also known as BIRC proteins). Human NAIP is 1403 amino acids (aa) in length. It contains three distinct regions: an N-terminal cluster of three baculoviral inhibitory repeat (BIR) domains, a central nucleotide binding oligomerization domain (NOD), and a C-terminal leucine-rich repeat (LRR) domain. Human NAIP shares 68% aa identity with mouse NAIP. NAIP is expressed in motor neurons, but not in sensory neurons. It is also expressed in the liver, placenta and to a lesser extent in the spinal cord. NAIP prevents motor neuron apoptosis, and defects in NAIP have been found in individuals with spinal muscular atrophy.

Long Name

Neuronal Apoptosis Inhibitor Protein

Alternate Names

BIRC1, NLRB1

Entrez Gene IDs

4671 (Human); 17940 (Mouse); 191568 (Rat)

Gene Symbol

NAIP

UniProt

NP_004527

Additional NAIP Products

Product Documents for Human NAIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NAIP Antibody

For research use only

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