Human Napsin A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8489
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1265A
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Napsin A
Thr25-Gly420
Accession # O96009
Thr25-Gly420
Accession # O96009
Specificity
Detects human Napsin A in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Napsin A Antibody
Detection of Human Napsin A by Western Blot.
Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Napsin A Monoclonal Antibody (Catalog # MAB8489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Napsin A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Napsin A in Human Kidney.
Napsin A was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Napsin A Monoclonal Antibody (Catalog # MAB8489) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Napsin A by Simple WesternTM.
Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Napsin A at approximately 46 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Napsin A Monoclonal Antibody (Catalog # MAB8489) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Napsin A Antibody
Application
Recommended Usage
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Simple Western
5 µg/mL
Sample: Human lung tissue
Sample: Human lung tissue
Western Blot
0.5 µg/mL
Sample: Human lung tissue and human kidney tissue
Sample: Human lung tissue and human kidney tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Napsin A
Alternate Names
ASP4, KAP, NAPA, NAPSA, Napsin-1, SNAPA, TA01/TA02
Gene Symbol
NAPSA
UniProt
Additional Napsin A Products
Product Documents for Human Napsin A Antibody
Product Specific Notices for Human Napsin A Antibody
For research use only
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