Human Napsin A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8489
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Dot Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Napsin A
Thr25-Gly420
Accession # O96009
Thr25-Gly420
Accession # O96009
Specificity
Detects human Napsin A in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Napsin A Antibody
Detection of Human Napsin A by Western Blot.
Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Napsin A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Napsin A in Human Kidney.
Napsin A was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Napsin A by Simple WesternTM.
Simple Western lane view shows lysates of human kidney, loaded at 0.2 mg/mL. A specific band was detected for Napsin A at approximately 45 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Napsin A Antibody
Application
Recommended Usage
Immunohistochemistry
0.5-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Simple Western
0.5 µg/mL
Sample: Human Kidney
Sample: Human Kidney
Western Blot
1:1000 dilution
Sample: Human lung tissue and human kidney tissue
Sample: Human lung tissue and human kidney tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Napsin A
Alternate Names
ASP4, KAP, NAPA, NAPSA, Napsin-1, SNAPA, TA01/TA02
Gene Symbol
NAPSA
UniProt
Additional Napsin A Products
Product Documents for Human Napsin A Antibody
Product Specific Notices for Human Napsin A Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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