Human Nectin-4 Antibody

Detection of Human Nectin-4/PVRL4 by Western Blot

Knockdown expression and over-expression of Nectin-4 in human EC cell lines Eca-109 and TE-1. a The knockdown expression of Nectin-4 at mRNA level by using RNAi approach in human EC lines was confirmed by real-time RT-PCR, and the Nectin-4 mRNA expression level in LV-Nectin-4-shRNA group cells was significantly lower than that in LV-NC group cells (both in Eca-109 and in TE-1, P < 0.0001). b The decreased Nectin-4 protein expression after knockdown in human EC cell lines Eca-109 and TE-1, were confirmed by using western blot method. c The Nectin-4 protein expression level in LV-Nectin-4-shRNA group cells was significantly lower than that in LV-NC group cells (in Eca-109, P < 0.01, and in TE-1, P < 0.001). d The over-expression of Nectin-4 at mRNA level in human EC lines was confirmed by real-time RT-PCR, which showed that the increased Nectin-4 mRNA expression level in LV-Nectin-4-OE group cells compared with LV-Vector-Ctrl group cells (both in Eca-109 and in TE-1, P < 0.0001). e The increased Nectin-4 protein expression in human EC cell lines Eca-109 and TE-1 were confirmed by using western blot method. f The Nectin-4 protein expression level in LV-Nectin-4-OE group cells was significantly higher than that in LV-Vector-Ctrl group cells (in Eca-109, P < 0.01, and in TE-1, P < 0.001) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Nectin-4/PVRL4 by Immunohistochemistry

Nectin-4 expression in EC tissues. a, b Positive staining of Nectin-4 could be found in the cytoplasm and on the membrane of cancer cells, while weak or negative staining of Nectin-4 was found in normal esophageal tissues. c The staining intensity of Nectin-4 in EC tissues was significantly higher than that in adjacent normal tissues (P < 0.0001). d The survival analysis showed that the overall survival rate of the patients with higher Nectin-4 expression was significantly poorer compared with those showing lower Nectin-4 expression (HR = 1.704, 95% CI 1.027–2.825, P = 0.039) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Nectin-4/PVRL4 by Immunohistochemistry

Nectin-4 expression in EC tissues. a, b Positive staining of Nectin-4 could be found in the cytoplasm and on the membrane of cancer cells, while weak or negative staining of Nectin-4 was found in normal esophageal tissues. c The staining intensity of Nectin-4 in EC tissues was significantly higher than that in adjacent normal tissues (P < 0.0001). d The survival analysis showed that the overall survival rate of the patients with higher Nectin-4 expression was significantly poorer compared with those showing lower Nectin-4 expression (HR = 1.704, 95% CI 1.027–2.825, P = 0.039) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Nectin-4/PVRL4 by Immunocytochemistry/Immunofluorescence

Nectin-4 expression in human pancreatic cancer tissues. (A,B) Tumor cell with high or low Nectin-4 expression. (C) There was limited expression of Nectin-4 in non-cancer tissues including islet cells, acinar cells and normal gland. Representative case of tumor Nectin-4 expression. Original magnification, ×200. Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0144-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Nectin-4/PVRL4 by Immunocytochemistry/Immunofluorescence

Nectin-4 expression in human pancreatic cancer tissues. (A,B) Tumor cell with high or low Nectin-4 expression. (C) There was limited expression of Nectin-4 in non-cancer tissues including islet cells, acinar cells and normal gland. Representative case of tumor Nectin-4 expression. Original magnification, ×200. Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0144-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Nectin-4/PVRL4 by Western Blot

Knockdown expression and over-expression of Nectin-4 in human EC cell lines Eca-109 and TE-1. a The knockdown expression of Nectin-4 at mRNA level by using RNAi approach in human EC lines was confirmed by real-time RT-PCR, and the Nectin-4 mRNA expression level in LV-Nectin-4-shRNA group cells was significantly lower than that in LV-NC group cells (both in Eca-109 and in TE-1, P < 0.0001). b The decreased Nectin-4 protein expression after knockdown in human EC cell lines Eca-109 and TE-1, were confirmed by using western blot method. c The Nectin-4 protein expression level in LV-Nectin-4-shRNA group cells was significantly lower than that in LV-NC group cells (in Eca-109, P < 0.01, and in TE-1, P < 0.001). d The over-expression of Nectin-4 at mRNA level in human EC lines was confirmed by real-time RT-PCR, which showed that the increased Nectin-4 mRNA expression level in LV-Nectin-4-OE group cells compared with LV-Vector-Ctrl group cells (both in Eca-109 and in TE-1, P < 0.0001). e The increased Nectin-4 protein expression in human EC cell lines Eca-109 and TE-1 were confirmed by using western blot method. f The Nectin-4 protein expression level in LV-Nectin-4-OE group cells was significantly higher than that in LV-Vector-Ctrl group cells (in Eca-109, P < 0.01, and in TE-1, P < 0.001) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Nectin-4/PVRL4 by Immunocytochemistry/Immunofluorescence

Nectin-4 expression in human pancreatic cancer tissues. (A,B) Tumor cell with high or low Nectin-4 expression. (C) There was limited expression of Nectin-4 in non-cancer tissues including islet cells, acinar cells and normal gland. Representative case of tumor Nectin-4 expression. Original magnification, ×200. Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0144-7), licensed under a CC-BY license. Not internally tested by R&D Systems.