Human NEDD9/CASL Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5459
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NEDD9
Lys153-Asn366
Accession # Q14511
Lys153-Asn366
Accession # Q14511
Specificity
Detects endogenous human NEDD9 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human NEDD9/CASL Antibody
Detection of Human NEDD9 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human NEDD9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5459) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NEDD9 at approximately 105 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human NEDD9/CASL Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and MCF-7 human breast cancer cell line
Sample: Daudi human Burkitt's lymphoma cell line and MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NEDD9/CASL
NEDD9 (also HEF1 and Cas-L) is a 93 kDa (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin beta1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834 amino acids (aa) in length and contains one SH3 domain (aa 3‑65), a Tyr-rich SH2-binding region (aa 90‑350), one Ser-rich four alpha-helix bundle protein interaction site (aa 350‑650), and an HLH motif that mediates Cas protein dimerization (aa 710‑760). NEDD9 usually runs at 105 or 115 kDa in SDS‑PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55 kDa fragment. One isoform shows a deletion of aa 155‑188, while two others show 20 and seven aa substitutions for aa 155‑834 and 1‑153, respectively. Over aa 153‑366, human and mouse NEDD9 share 85% aa identity.
Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9
Alternate Names
CAS2, CASL, HEF1, p105
Gene Symbol
NEDD9
UniProt
Additional NEDD9/CASL Products
Product Documents for Human NEDD9/CASL Antibody
Product Specific Notices for Human NEDD9/CASL Antibody
For research use only
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