NTera-2 human testicular embryonic carcinoma cell line either untreated (dark orange filled histogram) or treated with 5 µM Retinoic Acid for 6 days (light orange filled histogram) was stained with Mouse Anti-Human Nestin PE-conjugated Monoclonal Antibody (Catalog # IC1259P) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Nestin PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line either untreated or treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nestin

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

References

Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
Lendahl, U. et al. (1990) Cell 60:585.
Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
Frederiksen, K. et al. (1988) Neuron 1:439.
Cattaneo, E. et al. (1990) Nature 347:762.
Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
Rietze, R.L. et al. (2001) Nature 412:736.
Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
Zulewski, H. et al. (2001) Diabetes 50:521.
Lumelsky, N. et al. (2001) Science 292:1389.
Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
Shih, C.C. et al. (2001) Blood 98:2412.

Alternate Names

NES

Entrez Gene IDs

10763 (Human); 18008 (Mouse); 25491 (Rat)

Gene Symbol

NES

Additional Nestin Products

Product Documents for Human Nestin PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Nestin PE-conjugated Antibody

For research use only

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Flow Cytometry

Human Nestin PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1259P

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Nestin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Nestin PE-conjugated Antibody

Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry.