Human NF2/Merlin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5616
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 534428
Product Summary for Human NF2/Merlin Antibody
Immunogen
E. coli-derived recombinant human NF2/Merlin
Met363-Lys578
Accession # P35240
Met363-Lys578
Accession # P35240
Specificity
Detects human NF2/Merlin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NF1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human NF2/Merlin Antibody
Detection of Human NF2/Merlin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human NF2/Merlin Monoclonal Antibody (Catalog # MAB5616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NF2/Merlin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human NF2/Merlin Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC)
Sample: HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB5616 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NF2/Merlin
Long Name
Neurofibromin 2
Alternate Names
ACN, BANF, Merlin, SCH, Schwannomerlin, Schwannomin
Gene Symbol
NF2
UniProt
Additional NF2/Merlin Products
Product Documents for Human NF2/Merlin Antibody
Product Specific Notices for Human NF2/Merlin Antibody
For research use only
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