Human NFATC2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6499

R&D Systems, part of Bio-Techne
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MAB6499
MAB6499-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 639402

View all NFATC2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NFATC-2
His575-Pro679
Accession # Q13469

Specificity

Detects human NFATC2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human NFATC1 or C3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NFATC2 Antibody

Detection of Human NFATC2 antibody by Western Blot.

Detection of Human NFATC2 by Western Blot.

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Human NFATC2 Monoclonal Antibody (Catalog # MAB6499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NFATC2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human NFATC2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: BJAB human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and MOLT‑4 human acute lymphoblastic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC2

NFATC2 (Nuclear factor of activated T cells C2; also NFAT1 or NFAT2P) is a 135 kDa member of the NFAT family of transcription factors. NFATC2 is found in T cells and mast cells where it regulates cytokine transcription, Th2 cell differentiation, and cell cycle entry. The transactivation function of NFATC2 is regulated by phosphorylation at Ser53, Ser56, Thr116, and Ser170. Human NFATC2 is 925 amino acids (aa) in length. It contains a calcineurin-binding site (aa 111-116), a transactivation domain (aa 119-199), two NLS (aa 251-253 and 664-666), an RHD that binds DNA (aa 392-574), and one NES (aa 904-913). An alternate splice form has a substitution of the C-terminal 18 amino acids. Human NFATC2 shares 96% aa identity with mouse and rat NFATC2.

Long Name

Nuclear factor of activated T-cells, cytoplasmic 2

Alternate Names

NFAT1, NFATP

Entrez Gene IDs

4773 (Human)

Gene Symbol

NFATC2

UniProt

Q13469

Additional NFATC2 Products

Product Documents for Human NFATC2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NFATC2 Antibody

For research use only

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