Human NFATC3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5834
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 646115
Product Specifications
Immunogen
E. coli-derived recombinant human NFATC3
Asp900-Asp1035
Accession # Q12968
Asp900-Asp1035
Accession # Q12968
Specificity
Detects human NFATC3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human NFATC3 Antibody
Detection of Human NFATC3 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human NFATC3 Monoclonal Antibody (Catalog # MAB5834) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NFATC3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human NFATC3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NFATC3
Long Name
Nuclear Factor of Activated T Cells C3
Alternate Names
NFAT4, NFATX
Gene Symbol
NFATC3
UniProt
Additional NFATC3 Products
Product Documents for Human NFATC3 Antibody
Product Specific Notices for Human NFATC3 Antibody
For research use only
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