Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8570) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Applications for Human NFIL3/E4BP4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFIL3/E4BP4

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

References

Seillet C, et al (2014) J Exp Med. 211:1733.
Geiger TL, et al (2014) J Exp Med. 211:1723.

Long Name

Nuclear Factor, Interleukin 3 Regulated

Alternate Names

E4BP4, IL3BP1, NF-IL3A

Entrez Gene IDs

4783 (Human); 18030 (Mouse); 114519 (Rat)

Gene Symbol

NFIL3

UniProt

Q16649

Additional NFIL3/E4BP4 Products

Product Documents for Human NFIL3/E4BP4 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human NFIL3/E4BP4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human NFIL3/E4BP4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8570

Key Product Details

Validated by

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all NFIL3/E4BP4 Antibodies »

Product Summary for Human NFIL3/E4BP4 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human NFIL3/E4BP4 Antibody

Detection of NFIL3/E4BP4 in Human PBMCs by Flow Cytometry.