Human Nicastrin Isoform 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5378
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Nicastrin Isoform 1
Asn34-Glu669
Accession # Q92542
Asn34-Glu669
Accession # Q92542
Specificity
Detects human Nicastrin Isoform 1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Nicastrin Isoform 1 Antibody
Detection of Human Nicastrin Isoform 1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Nicastrin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5378) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nicastrin Isoform 1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Nicastrin in Human Alzheimer's Disease Brain.
Nicastrin was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex) using 1.7 µg/mL Sheep Anti-Human Nicastrin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5378) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Nicastrin Isoform 1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex)
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and human liver tissue
Sample: HepG2 human hepatocellular carcinoma cell line and human liver tissue
Reviewed Applications
Read 3 reviews rated 3.7 using AF5378 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nicastrin
Nicastrin (NCT) is a 150‑160 kDa member of the nicastrin family of proteins. It is a component of the aspartyl protease gamma-secretase complex and serves to stabilize and direct gamma-secretase components to proper positions in the plasma membrane. The gamma-secretase complex mediates the cleavage of intramembrane proteins such as notch-1 and APP. Mature human nicastrin is a 676 amino acid type I transmembrane glycoprotein. It contains a 636 aa extracellular domain (aa 34‑669) that shows a 58 aa sequence (aa 312‑369) which interacts with gamma-secretase substrates. There are multiple splice variants of NCT. One shows a deletion of aa 195‑322 and 394‑709, a second shows a 29 aa substitution for the C-terminal 604 aa and a third shows a deletion of aa 200‑709 accompanied by an insertion of 33 aa after Leu30. Over aa 34‑669, human NCT shares 90% aa identity with mouse NCT.
Alternate Names
APH2, NCSTN
Gene Symbol
NCSTN
UniProt
Additional Nicastrin Products
Product Documents for Human Nicastrin Isoform 1 Antibody
Product Specific Notices for Human Nicastrin Isoform 1 Antibody
For research use only
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