HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP was stained with either (A) Mouse Anti-Human NKCC1/SLC12A2 Monoclonal Antibody (Catalog # MAB8528) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human NKCC1/SLC12A2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKCC1/SLC12A2

Solute Carrier, family 12 (potassium/chloride transporter), member 2 (SLC12A2) encodes NKCC1, which mediates sodium and chloride transport and reabsorption. It is a membrane protein that is important in maintaining proper ionic balance and cell volume. In contrast to the renal-specific sodium/potassium/chloride cotransporter SLC12A1, SLC12A2 is expressed in many tissues, including the basolateral membrane of secretory epithelia. It is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for SLC12A2.

Long Name

Sodium/Potassium/Chloride Transporter NKCC1

Alternate Names

BSC2, SLC12A2

Entrez Gene IDs

6558 (Human); 20496 (Mouse); 83629 (Rat)

Gene Symbol

SLC12A2

UniProt

P55011

Additional NKCC1/SLC12A2 Products

Product Documents for Human NKCC1/SLC12A2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human NKCC1/SLC12A2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human NKCC1/SLC12A2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8528

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all NKCC1/SLC12A2 Antibodies »

Product Summary for Human NKCC1/SLC12A2 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human NKCC1/SLC12A2 Antibody

Detection of NKCC1/SLC12A2 in HEK293 Human Cell Line Transfected with Human NKCC1/SLC12A2 and eGFP by Flow Cytometry.