HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP was stained with either (A) Mouse Anti-Human NKCC1/SLC12A2 APC-conjugated Monoclonal Antibody (Catalog # FAB8528A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NKCC1/SLC12A2 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKCC1/SLC12A2

Solute Carrier, family 12 (potassium/chloride transporter), member 2 (SLC12A2) encodes NKCC1, which mediates sodium and chloride transport and reabsorption. It is a membrane protein that is important in maintaining proper ionic balance and cell volume. In contrast to the renal-specific sodium/potassium/chloride cotransporter SLC12A1, SLC12A2 is expressed in many tissues, including the basolateral membrane of secretory epithelia. It is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for SLC12A2.

Long Name

Sodium/Potassium/Chloride Transporter NKCC1

Alternate Names

BSC2, SLC12A2

Entrez Gene IDs

6558 (Human); 20496 (Mouse); 83629 (Rat)

Gene Symbol

SLC12A2

UniProt

P55011

Additional NKCC1/SLC12A2 Products

Product Documents for Human NKCC1/SLC12A2 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human NKCC1/SLC12A2 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human NKCC1/SLC12A2 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8528A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NKCC1/SLC12A2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human NKCC1/SLC12A2 APC-conjugated Antibody

Detection of NKCC1/SLC12A2 in HEK293 Human Cell Line Transfected with Human NKCC1/SLC12A2 and eGFP by Flow Cytometry.