Human NOMO Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3755

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AF3755
AF3755-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all NOMO Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NOMO
Ser32- Asp1155
Accession # Q15155

Specificity

Detects human NOMO in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human NOMO Antibody

Detection of Human NOMO antibody by Western Blot.

Detection of Human NOMO by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human NOMO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NOMO at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human NOMO Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NOMO

NOMO, also known as pM5, is a 130 kDa type I membrane protein with no recognizable functional motif. It is a binding partner of Nicalin, a nicastin-related protein. The Nicalin/NOMO complex is localized in the ER and functions as an antagonist for Nodal and Activin signaling. Three copies of the NOMO gene, encoding NOMO isoforms 1, 2 and 3 have been described. The extracellular domains (ECD) of the isoforms share 99% amino acid sequence homology. The ECD of human and mouse NOMO also share 94% amino acid sequence homology.

Long Name

NODAL Modulator

Alternate Names

NOMO2, PM5

Entrez Gene IDs

283820 (Human)

Gene Symbol

NOMO2

UniProt

Q15155

Additional NOMO Products

Product Documents for Human NOMO Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NOMO Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.