Human NTH1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2675
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 208521
Product Specifications
Immunogen
E. coli-derived recombinant human NTH1
Met1-Leu312
Accession # P78549
Met1-Leu312
Accession # P78549
Specificity
Detects human NTH1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human NTH1 Antibody
Detection of Human NTH1 by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human NTH1 Monoclonal Antibody (Catalog # MAB2675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for NTH1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human NTH1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NTH1
NTH1 is a DNA glycosylase with apurinic lyase activity. It functions in the repair of oxidative damage to DNA through the BER (base excision repair) pathway.
Long Name
Nth Endonuclease III-like 1
Alternate Names
NTHL1, OCTS3
Entrez Gene IDs
4913 (Human)
Gene Symbol
NTHL1
UniProt
Additional NTH1 Products
Product Documents for Human NTH1 Antibody
Product Specific Notices for Human NTH1 Antibody
For research use only
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