Human Olig 1, 2, 3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2230
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry
Cited:
Bioassay, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 257224
Product Specifications
Immunogen
E. coli-derived recombinant human Olig1
aa 1-272
aa 1-272
Specificity
Detects human Olig 1, 2, 3. In direct ELISAs, this antibody shows approximately 20% cross‑reactivity with recombinant human (rh) Olig2 and 50 - 100% cross‑reactivity with rhOlig3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Olig 1, 2, 3 Antibody
Olig 1, 2, 3 in Mouse Embryo.
Olig 1, 2, 3 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Mouse Anti-Human Olig 1, 2, 3 Monoclonal Antibody (Catalog # MAB2230) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neurons in developing brain. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Olig 1, 2, 3 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line
Sample: Immersion fixed A172 human glioblastoma cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo.
Sample: Immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Olig 1, 2, 3
Long Name
Oligodendrocyte Lineage Gene
Additional Olig 1, 2, 3 Products
Product Documents for Human Olig 1, 2, 3 Antibody
Product Specific Notices for Human Olig 1, 2, 3 Antibody
For research use only
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