Human Olig1, 2, 3 PE‑conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC2230P
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 257224
Product Specifications
Immunogen
E. coli-derived recombinant human Olig1
aa 1-272
aa 1-272
Specificity
Detects human Olig1, 2, 3. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) Olig2 and 50-100% cross-reactivity with rhOlig3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Olig1, 2, 3 PE‑conjugated Antibody
Detection of Olig1, 2, 3 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Mouse Anti-Human Olig1, 2, 3 PE-conjugated Monoclonal Antibody (Catalog # IC2230P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human Olig1, 2, 3 PE‑conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: A172 human glioblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: A172 human glioblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Olig 1, 2, 3
Long Name
Oligodendrocyte Lineage Gene
Additional Olig 1, 2, 3 Products
Product Documents for Human Olig1, 2, 3 PE‑conjugated Antibody
Product Specific Notices for Human Olig1, 2, 3 PE‑conjugated Antibody
For research use only
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