Human p21/CIP1/CDKN1A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1047
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human p21
Ser2-Pro164
Accession # P38936
Ser2-Pro164
Accession # P38936
Specificity
Detects human p21 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human p21/CIP1/CDKN1A Antibody
Detection of Human p21/CIP1/CDKN1A by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p21/CIP1/CDKN1A at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p21/CIP1/CDKN1A in MCF‑7 Human Cell Line.
p21/CIP1/CDKN1A was detected in immersion fixed MCF-7 human breast cancer cell line treated with (left panel) or without (right panel) camptothecin using Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
p21/CIP1/CDKN1A in Human Breast Cancer Tissue.
p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 1.7 µg/mL Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human p21/CIP1/CDKN1A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line treated with Camptothecin
Sample: Immersion fixed MCF-7 human breast cancer cell line treated with Camptothecin
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Immunoprecipitation
1-2 µg/500 µg cell lysate
Sample: MCF-7 human breast cancer cell line, see our available Western blot detection antibodies
Sample: MCF-7 human breast cancer cell line, see our available Western blot detection antibodies
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: MCF-7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with methanol
Sample: MCF-7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with methanol
Simple Western
5 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Camptothecin (CPT)
Sample: MCF‑7 human breast cancer cell line treated with Camptothecin (CPT)
Western Blot
0.5 µg/mL
Sample: Camptothecin-treated MCF-7 human breast cancer cell line
Sample: Camptothecin-treated MCF-7 human breast cancer cell line
Reviewed Applications
Read 4 reviews rated 4.3 using AF1047 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p21/CIP1/CDKN1A
Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Alternate Names
CDKN1A, CIP1
Gene Symbol
CDKN1A
UniProt
Additional p21/CIP1/CDKN1A Products
Product Documents for Human p21/CIP1/CDKN1A Antibody
Product Specific Notices for Human p21/CIP1/CDKN1A Antibody
For research use only
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