Human p38 delta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1519
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human p38 delta
Accession # O15264
Accession # O15264
Specificity
Detects endogenous human p38 delta. Mouse and rat p38 delta reactivity has not been demonstrated but is likely due to their high homology with human p38 delta. This antibody does not detect recombinant p38 alpha, p38 beta or p38 gamma in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human p38 delta Antibody
Detection of Human p38 delta by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human p38 delta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1519) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, recombinant p38 beta, p38 gamma, p38 delta, and p38 alpha at 2 ng/lane) were included. A specific band for p38 delta was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p38 delta in Human Skin.
p38d was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Rabbit Anti-Human p38d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1519) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p38 delta in Human Skin.
p38d was detected in immersion fixed paraffin-embedded sections of human skin using Rabbit Anti-Human p38d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1519) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human p38 delta Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Sample: Immersion fixed paraffin-embedded sections of human skin
Western Blot
0.1 µg/mL
Sample: HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line
Sample: HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using AF1519 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 delta
The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses. All four p38 family members, alpha, beta, gamma, and delta, are phosphorylated by MKK3 and/or MKK6 at dual Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Once activated, p38 phosphorylates a number of targets, including the nuclear transcription factors ATF2 and Max.
The most frequently analyzed family member, p38 alpha, also known as SAPK2a and MAPK14, was initially purified as a kinase critical to the signaling cascade linking IL-1 to MAPKAPK-2 and the small heat shock protein HSP27. Ubiquitously expressed, p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the cytoplasmic kinases MNK 4 and PRAK5 and the nuclear transcription factors ATF2 1 and STAT1. Several promising compounds that inhibit p38 alpha are being investigated as potential therapies for arthritic and inflammatory diseases.
Additional p38 delta Products
Product Documents for Human p38 delta Antibody
Product Specific Notices for Human p38 delta Antibody
For research use only
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