Human Palladin Antibody

Detection of Human Palladin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, PC-3 human prostate cancer cell line, and Caki-2 human clear cell carcinoma epithelial cell line. PVDF Membrane was probed with 0.5 µg/mL of Human Palladin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5950) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). Specific bands were detected for Palladin at approximately 95, 115, and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Palladin in Human Prostate.

Palladin was detected in immersion fixed paraffin-embedded sections of Human Prostate using Sheep Anti-Human Palladin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5950) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.