Human Pancreatic Polypeptide/PP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6297
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 548301
Product Specifications
Immunogen
E. coli-derived recombinant human Pancreatic Polypeptide/PP
Ala30-Leu95
Accession # P01298
Ala30-Leu95
Accession # P01298
Specificity
Detects Pancreatic Polypeptide/PP in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Pancreatic Polypeptide/PP Antibody
Detection of Human Pancreatic Polypeptide by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 2 µg/mL of Human Pancreatic Polypeptide Monoclonal Antibody (Catalog # MAB6297) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pancreatic Polypeptide at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Pancreatic Polypeptide/PP Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human pancreas tissue
Sample: Human pancreas tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pancreatic Polypeptide/PP
Pancreatic Polypeptide (PP) is an 11 kDa (calculated), unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence. PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake. The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.
Additional Pancreatic Polypeptide/PP Products
Product Documents for Human Pancreatic Polypeptide/PP Antibody
Product Specific Notices for Human Pancreatic Polypeptide/PP Antibody
For research use only
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