Human Pax7 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6670
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Pax7
Met1-Gly196
Accession # P23759
Met1-Gly196
Accession # P23759
Specificity
Detects human Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Pax3 is observed and approximately 4% cross-reactivity with rhPax1 and rhPax6 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Pax7 Antibody
Detection of Human Pax7 by Western Blot.
Western blot shows lysates of human muscle tissue. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human Pax7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6670) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pax7 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Pax7 in TT Cell Line (Positive) and THP-1 Cell Line (Negative).
Pax7 was detected in immersion fixed TT Human Medullary Thyroid Cancer Cell Line (Positive) and absent in THP-1 Human Acute Monocytic Leukemia Cell Line (Negative) using Goat Anti-Human Pax7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6670) at 5 µg/mL for 1 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Pax7 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed TT Human Medullary Thyroid Cancer Cell Line (Positive) and THP-1 Human Acute Monocytic Leukemia Cell Line (Negative)
Sample: Immersion fixed TT Human Medullary Thyroid Cancer Cell Line (Positive) and THP-1 Human Acute Monocytic Leukemia Cell Line (Negative)
Western Blot
0.5 µg/mL
Sample: Human muscle tissue
Sample: Human muscle tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax7
PAX7 (Paired box 7A; also HuP1) is a 57 kDa member of the paired homeobox family of transcription factors. It is expressed in select neurons (in rodent) and skeletal muscle satellite cells, where, in the latter, it promotes both a muscle stem cell state (PAX7+ desmin- cells) and a lineage commitment state (PAX7+ desmin+ cells). PAX7 forms heterodimers with PAX3, and interacts with the regulatory regions of the Myf5 locus. Human PAX7(A) is 520 amino acids (aa) in length. It contains an N‑terminal paired box DNA-binding domain (aa 34-163), a homeodomain (aa 218-275) and a C-terminal poly-alanine region (aa 340-346). Multiple splice forms of PAX7 exist. There is a 54 kDa isoform B that contains a 38 aa substitution for aa 468-520, plus a second isoform that shows a deletion of Gly151 and Leu152. There is also a chromosomal rearrangement that fuses aa 30-385 of PAX7 to aa 211-265 of FOXO1A/FKHR. Over aa 1-196, human PAX7 shares 86% and 96% aa identity with human PAX3 and mouse PAX7, respectively.
Long Name
Paired Box Gene 7
Alternate Names
HUP1, RMS2
Gene Symbol
PAX7
UniProt
Additional Pax7 Products
Product Documents for Human Pax7 Antibody
Product Specific Notices for Human Pax7 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...